Subdermal Bloodless Space and Prolongation of Collagen in Rats

Li Nguyen^1,5*, Hanh Nguyen², Thanh Phung³ and Joel Sercarz^4,6

¹Department of Otolaryngology/Head and Neck Surgery, Automatic Subdermal Injection System, Inc, 15751 Brookhurst Ste 105, Westminster, California 92683, U.S.A.
²Department of Radiology,Magnolia Medical Imaging Center 1, 14571 Magnolia Ste 101, Westminster, California 92683, U.S.A.
³Department of Radiology, Magnolia Medical Imaging Center 2, 22921 Triton Way Ste 125, Laguna Hills, California 92653, U.S.A.
⁴Department of Radiology,Magnolia Medical Imaging Center 3, 1111 W Town and Country Ste 30, Orange, California 92868, U.S.A.
⁵Department of Anesthesiology, Innovative Drug Injection Technology Inc., 881 Dover Dr. Ste 350, Newport Beach, California 92663, U.S.A.
⁶Department of Otolaryngology/Head and Neck Surgery,University of California, Los Angeles, Medical Plaza, Suite 550, Los Angeles, California 90095, U.S.A.

Accepted May 13, 2014

Products injected into the subdermal bloodless space imply longer lasting medicinal effects and avoidance of initial toxicity and side effects immediately following injection. This is desirable particularly for cosmetic products such as dermal fillers or BOTOX® since they have no reason to travel to the rest of the body. In this study, we have found dermal filler Zyplast®(bovine collagen) remained for at least twelve weeks post-injection in four out of four rat shoulders when injected into subdermal bloodless space, whereas, Zyplast® randomly injected disappeared betweentwo and twelve weekspost-injection in four out of four rat buttocks. Manual blind injections of Zyplast® could only be successfully injected into the subdermal bloodless space 50% of the time at best; wehad greater success by injecting Zyplast® using the Automated Subdermal Injection System technique.

Keywords: Collagen, electrical stimulation, injectable EMG needle, subdermal bloodless space, subdermal injection, Zyplast.

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